Abstract: The respiratory research comprehensive medical center project has the characteristics of multiple functions and miscellaneous functions, and is highly professional. In order to study the new technology of design and construction of this kind of project, taking a respiratory medical center project in Guangzhou as an example and taking the project construction process as the background, the professional functions of the respiratory research comprehensive medical center are introduced in the preliminary design, and the professional functions are realized through engineering construction. In the construction process, the design and construction are deeply integrated, and the functions of various medical specialties are gradually improved. Finally, the research content and innovation points of the construction process are summarized to form a comprehensive new technology for the design and construction of such projects. The main innovative technologies are described in the paper as a whole. Focus on the anti-infection technology, anti-radiation technology, intelligent technology, sponge city technology, rapid construction technology and other contents are introduced in detail, which is the innovative practice of this kind of comprehensive project with hospitals, training bases, medical transformation and basic experiments in one. After the application of relevant new technologies and new processes, good results have been achieved.