Abstract: This study is based on the jack-pushing project of the steel-concrete composite continuous beam bridge (main span of 65 meters) over the Kaida Road crossing the Guangzhou-Shenzhen Expressway. It aims to accurately grasp the mechanical properties and structural safety of the steel-concrete composite beam bridge during the jack-pushing construction process, thereby ensuring construction accuracy. Firstly, based on the Midas/Civil finite element software, a two-dimensional and three-dimensional model of the entire bridge considering the voids in temporary supports (simulated using only compression-only elastic supports) and equivalent loads of the poured bridge deck was established. Eight working conditions were divided according to the construction plan for simulation analysis, and the key section positions of maximum positive and negative bending moments during the jack-pushing process were identified. Subsequently, for the identified key sections, a detailed real-time stress monitoring plan was formulated: six vibrating wire strain gauge measuring points were arranged on each section, and a Datataker DT85G data acquisition instrument was used, combined with wireless bridge transmission technology and a mobile power supply independent power supply system, to develop a high-stability, low-latency (<200 ms) remote real-time monitoring plan. The monitoring data analysis showed that the steel box girder had good integrity, uniform stress distribution, and no local buckling phenomenon; the section deformation conformed to the plane section assumption, and the structure was in a linear elastic state. The study also found that significant non-uniform temperature gradients caused by sunlight (such as the strain peak value of 40.2 µε at the upper measuring point being 39.5% higher than that at the lower measuring point, and the strain amplitude at the left side being 9.8% higher than that at the right side during the monitoring period) led to stress redistribution in the structure and may generate additional bending moments and torques, causing the beam alignment to deviate from the design position. To improve the accuracy of the jack-pushing construction, it is recommended to carry out multi-source monitoring and numerical simulation collaborative control.