Abstract: In response to the technical challenges of insufficient compressive performance and significant thermal expansion effect of acrylic transparent panels in large-span fish pond roof projects, this article relies on a museum's aerial fish pond project to systematically improve the structural safety and environmental adaptability of acrylic fish pond roofs through material performance testing, finite element simulation, and construction technology innovation. Specifically, this article conducted mechanical properties and thermal parameter tests on irregular acrylic sheets, and the results showed that the material had a tensile strength of 70.9 MPa, a light transmittance of 91.3%, and a linear expansion coefficient of 6.6 × 10⁻⁵/℃. Through finite element simulation method, the load combination conditions were analyzed, and the results showed that the maximum displacement of the acrylic transparent plate was 6.171 mm (L/2500), and the peak stress was 1.948 N/mm², far below its allowable stress. In addition, this article also adopts an integrated technology system, including an F-clamp square tube lifting system (with a safety factor of 136%), a thermal expansion compensation node (capable of absorbing 58.1 mm deformation), and a "three cloth and five coating" waterproof process (achieving zero leakage in water pressure testing). Engineering practice has shown that this technology system can effectively solve the deformation coordination and construction difficulties of large-span acrylic transparent roofs, significantly improving the durability of the structure and the reliability of construction. At the same time, engineering practice has further verified the applicability of this technology system in large-span transparent fish pond roofs, which can provide a technical paradigm for similar projects.