Abstract: The issue of human comfort caused by the flexibility of super high-rise steel structures has become an urgent engineering challenge that needs to be addressed. This study, based on a real-world super-tall frame-shear wall steel structure project, conducted wind tunnel tests to analyze unfavorable wind angles and wind load distribution patterns in super-tall steel structures. Using wind tunnel pressure measurement data, the structural wind-induced dynamic response was analyzed, with a focus on the acceleration response under the wind load of a once-in-a-year recurrence period at the most unfavorable wind angle. The research results indicate that the probability of wind load occurrence is highest within the 40° wind direction angle range, corresponding to a wind speed of 30 m/s. This wind direction angle is the most unfavorable for the structure. The fluctuating wind response of the structure under wind load is significant and closely related to the dynamic characteristics of the first three orders of the structure, indicating that the structure is a significantly wind-sensitive flexible structure. Under the once-in-a-year recurrence wind load at the most unfavorable wind angle (40°), the peak acceleration at the measurement point on the 33rd floor of the structure reached 0.319 m/s², 2.9 times the acceleration limit (0.11 m/s²). This demonstrates that the wind-induced acceleration response is substantial, and the structure alone cannot meet the comfort design requirements for wind-induced vibrations. Additional damping or optimized components are necessary to enhance structural stiffness and improve wind-induced comfort.